Syringomycin production among strains of Pseudomonas syringae pv. syringae: conservation of the syrB and syrD genes and activation of phytotoxin production by plant signal molecules.
Identifieur interne : 000292 ( Main/Exploration ); précédent : 000291; suivant : 000293Syringomycin production among strains of Pseudomonas syringae pv. syringae: conservation of the syrB and syrD genes and activation of phytotoxin production by plant signal molecules.
Auteurs : N B Quigley ; D C GrossSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ]
Descripteurs français
- KwdFr :
- ADN bactérien (analyse), ADN bactérien (métabolisme), Cartographie de restriction (MeSH), Données de séquences moléculaires (MeSH), Gènes bactériens (MeSH), Phénomènes physiologiques des plantes (MeSH), Plantes (microbiologie), Plasmides (MeSH), Polymorphisme de restriction (MeSH), Protéines bactériennes (biosynthèse), Protéines bactériennes (génétique), Protéines de transport (biosynthèse), Protéines de transport (génétique), Pseudomonas (génétique), Pseudomonas (métabolisme), Pseudomonas (pathogénicité), Séquence d'acides aminés (MeSH), Technique de Southern (MeSH), Toxines biologiques (biosynthèse), Transporteurs ABC (MeSH), Virulence (génétique).
- MESH :
- analyse : ADN bactérien.
- biosynthèse : Protéines bactériennes, Protéines de transport, Toxines biologiques.
- génétique : Protéines bactériennes, Protéines de transport, Pseudomonas, Virulence.
- microbiologie : Plantes.
- métabolisme : ADN bactérien, Pseudomonas.
- pathogénicité : Pseudomonas.
- Cartographie de restriction, Données de séquences moléculaires, Gènes bactériens, Phénomènes physiologiques des plantes, Plasmides, Polymorphisme de restriction, Séquence d'acides aminés, Technique de Southern, Transporteurs ABC.
English descriptors
- KwdEn :
- ATP-Binding Cassette Transporters (MeSH), Amino Acid Sequence (MeSH), Bacterial Proteins (biosynthesis), Bacterial Proteins (genetics), Blotting, Southern (MeSH), Carrier Proteins (biosynthesis), Carrier Proteins (genetics), DNA, Bacterial (analysis), DNA, Bacterial (metabolism), Genes, Bacterial (MeSH), Molecular Sequence Data (MeSH), Plant Physiological Phenomena (MeSH), Plants (microbiology), Plasmids (MeSH), Polymorphism, Restriction Fragment Length (MeSH), Pseudomonas (genetics), Pseudomonas (metabolism), Pseudomonas (pathogenicity), Restriction Mapping (MeSH), Toxins, Biological (biosynthesis), Virulence (genetics).
- MESH :
- chemical , analysis : DNA, Bacterial.
- chemical , biosynthesis : Bacterial Proteins, Carrier Proteins, Toxins, Biological.
- chemical , genetics : Bacterial Proteins, Carrier Proteins.
- chemical , metabolism : DNA, Bacterial.
- chemical : ATP-Binding Cassette Transporters.
- genetics : Pseudomonas, Virulence.
- metabolism : Pseudomonas.
- microbiology : Plants.
- pathogenicity : Pseudomonas.
- Amino Acid Sequence, Blotting, Southern, Genes, Bacterial, Molecular Sequence Data, Plant Physiological Phenomena, Plasmids, Polymorphism, Restriction Fragment Length, Restriction Mapping.
Abstract
The syrB and syrD genes of Pseudomonas syringae pv. syringae are predicted to encode proteins that function in the synthesis and export of syringomycin, respectively. Using portions of the syr genes as DNA probes, both genes were shown to be conserved as single copies within a 15-kb or smaller DNA region among a broad spectrum of P. s. pv. syringae strains that produce syringomycin or one of its amino acid analogs, syringotoxin and syringostatin. Strains representative of P. viridiflava and six pathovars of P. syringae failed to hybridize with the gene probes, demonstrating that syr sequences are highly specific to P. s. pv. syringae and related nonpathogenic strains. Maximum parsimony analysis of restriction fragment length polymorphism profiles was used to evaluate relatedness among strains within the syrB and syrD gene region. A tree, conveying the smallest number of evolutionary changes among strains, revealed considerable diversity within the syr gene region; subclusters of strains were identified that appear to share specific qualities relevant to the plant-pathogen interaction. Because both the syrB gene and syringomycin production can be induced in response to plant signal molecules, 42 strains containing homologous syr sequences were tested for signal-mediated induction of toxin production. Over 90% of the toxigenic strains produced larger quantities of toxin when the plant signal molecules, arbutin and D-fructose, were added to syringomycin-minimal medium; 13 of the strains produced > or = 10-fold higher toxin levels. Some strains, such as 5D428, produced toxin only in the presence of these signals.(ABSTRACT TRUNCATED AT 250 WORDS)
DOI: 10.1094/mpmi-7-0078
PubMed: 7909458
Affiliations:
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(biosynthèse)</term><term>Transporteurs ABC (MeSH)</term><term>Virulence (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Bacterial Proteins</term><term>Carrier Proteins</term><term>Toxins, Biological</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Carrier Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>ATP-Binding Cassette Transporters</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN bactérien</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines bactériennes</term><term>Protéines de transport</term><term>Toxines biologiques</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pseudomonas</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines bactériennes</term><term>Protéines de transport</term><term>Pseudomonas</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Pseudomonas</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plants</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN bactérien</term><term>Pseudomonas</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Pseudomonas</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Pseudomonas</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Blotting, Southern</term><term>Genes, Bacterial</term><term>Molecular Sequence Data</term><term>Plant Physiological Phenomena</term><term>Plasmids</term><term>Polymorphism, Restriction Fragment Length</term><term>Restriction Mapping</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie de restriction</term><term>Données de séquences moléculaires</term><term>Gènes bactériens</term><term>Phénomènes physiologiques des plantes</term><term>Plasmides</term><term>Polymorphisme de restriction</term><term>Séquence d'acides aminés</term><term>Technique de Southern</term><term>Transporteurs ABC</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The syrB and syrD genes of Pseudomonas syringae pv. syringae are predicted to encode proteins that function in the synthesis and export of syringomycin, respectively. Using portions of the syr genes as DNA probes, both genes were shown to be conserved as single copies within a 15-kb or smaller DNA region among a broad spectrum of P. s. pv. syringae strains that produce syringomycin or one of its amino acid analogs, syringotoxin and syringostatin. Strains representative of P. viridiflava and six pathovars of P. syringae failed to hybridize with the gene probes, demonstrating that syr sequences are highly specific to P. s. pv. syringae and related nonpathogenic strains. Maximum parsimony analysis of restriction fragment length polymorphism profiles was used to evaluate relatedness among strains within the syrB and syrD gene region. A tree, conveying the smallest number of evolutionary changes among strains, revealed considerable diversity within the syr gene region; subclusters of strains were identified that appear to share specific qualities relevant to the plant-pathogen interaction. Because both the syrB gene and syringomycin production can be induced in response to plant signal molecules, 42 strains containing homologous syr sequences were tested for signal-mediated induction of toxin production. Over 90% of the toxigenic strains produced larger quantities of toxin when the plant signal molecules, arbutin and D-fructose, were added to syringomycin-minimal medium; 13 of the strains produced > or = 10-fold higher toxin levels. Some strains, such as 5D428, produced toxin only in the presence of these signals.(ABSTRACT TRUNCATED AT 250 WORDS)</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">7909458</PMID><DateCompleted><Year>1994</Year><Month>06</Month><Day>01</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>1</Issue><PubDate><MedlineDate>1994 Jan-Feb</MedlineDate></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Syringomycin production among strains of Pseudomonas syringae pv. syringae: conservation of the syrB and syrD genes and activation of phytotoxin production by plant signal molecules.</ArticleTitle><Pagination><MedlinePgn>78-90</MedlinePgn></Pagination><Abstract><AbstractText>The syrB and syrD genes of Pseudomonas syringae pv. syringae are predicted to encode proteins that function in the synthesis and export of syringomycin, respectively. Using portions of the syr genes as DNA probes, both genes were shown to be conserved as single copies within a 15-kb or smaller DNA region among a broad spectrum of P. s. pv. syringae strains that produce syringomycin or one of its amino acid analogs, syringotoxin and syringostatin. Strains representative of P. viridiflava and six pathovars of P. syringae failed to hybridize with the gene probes, demonstrating that syr sequences are highly specific to P. s. pv. syringae and related nonpathogenic strains. Maximum parsimony analysis of restriction fragment length polymorphism profiles was used to evaluate relatedness among strains within the syrB and syrD gene region. A tree, conveying the smallest number of evolutionary changes among strains, revealed considerable diversity within the syr gene region; subclusters of strains were identified that appear to share specific qualities relevant to the plant-pathogen interaction. Because both the syrB gene and syringomycin production can be induced in response to plant signal molecules, 42 strains containing homologous syr sequences were tested for signal-mediated induction of toxin production. Over 90% of the toxigenic strains produced larger quantities of toxin when the plant signal molecules, arbutin and D-fructose, were added to syringomycin-minimal medium; 13 of the strains produced > or = 10-fold higher toxin levels. Some strains, such as 5D428, produced toxin only in the presence of these signals.(ABSTRACT TRUNCATED AT 250 WORDS)</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Quigley</LastName><ForeName>N B</ForeName><Initials>NB</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Washington State University, Pullman 99164-6430.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gross</LastName><ForeName>D C</ForeName><Initials>DC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018528">ATP-Binding Cassette Transporters</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002352">Carrier Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014118">Toxins, Biological</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C084291">syrD protein, Pseudomonas syringae</NameOfSubstance></Chemical><Chemical><RegistryNumber>11140-67-3</RegistryNumber><NameOfSubstance UI="C032677">syringomycin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018528" MajorTopicYN="N">ATP-Binding Cassette Transporters</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015139" MajorTopicYN="N">Blotting, Southern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002352" MajorTopicYN="N">Carrier Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005798" MajorTopicYN="Y">Genes, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018521" MajorTopicYN="Y">Plant Physiological Phenomena</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010944" MajorTopicYN="N">Plants</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012150" MajorTopicYN="Y">Polymorphism, Restriction Fragment Length</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011549" MajorTopicYN="N">Pseudomonas</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015183" MajorTopicYN="N">Restriction Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014118" MajorTopicYN="N">Toxins, Biological</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1994</Year><Month>1</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1994</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1994</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">7909458</ArticleId><ArticleId IdType="doi">10.1094/mpmi-7-0078</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Gross, D C" sort="Gross, D C" uniqKey="Gross D" first="D C" last="Gross">D C Gross</name><name sortKey="Quigley, N B" sort="Quigley, N B" uniqKey="Quigley N" first="N B" last="Quigley">N B Quigley</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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